Know how

AYAWANE has one technological platform to develop new chemical entities as well as in vitro and ex vivo biological evaluations for screening and efficacy studies.
Our know-how is divided in three parts :

1-Chemical synthesis :

  • New chemical entities synthesis
  • Natural or synthetic polymers functionalization
  • Polysaccharides experts : hyaluronic acid, dextran, cellulose,...
  • Biomaterials/hydrogels elaboration (soluble and insoluble)
  • Green chemistry, ecofriendly

2-physicochemical characterization :

  • Structural analysis : NMR spectroscopy, infrared spectroscopy,...
  • Analytical methods : HPLC, GPC,...
  • Electrophoretic properties : agarose gel, acrylamide gel, western blot
  • Viscosity measurements
  • Stability assays

3-Biological screening :

  • Cell cytotoxicity : determination of IC50
  • Cell proliferation : determination of ED50
  • Cellular adhesion : soluble or coated molecules, biomaterials
  • Cellular migration : calibrated scratch assay
  • Dermal equivalent (FPLC) : lattice contraction, reorganization
  • ELISA assays : growth factors, cytokines, extracellular matrix components

To know more : Watch the services brochure (french)

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